Proper characterization and quality control are essential for ensuring the integrity, reliability, and safety of iPSC lines. Our comprehensive process includes:
| Cell Morphology | Evaluating growth patterns of typical iPSCs |
| Molecular Analysis | Confirming pluripotency-associated gene expression using PCR and RNA sequencing |
| Growth and Viability Assessment | Monitoring cell proliferation and health |
| Microbial and Fungal Sterility Testing | Detecting and eliminating contaminants |
| Mycoplasma Testing | Preventing contamination by mycoplasma species |
| Human Pathogen Testing | Screening for pathogens to ensure safety |
| Identity Testing | Microsatellite analysis for genetic match verification |
| Cytogenetic G-Banded Karyotype | Ensuring chromosomal stability |
| Microarray Genotyping | Confirming genetic authenticity and stability |
| Confirmation of Integration-Free Reprogramming | Verifying no genomic integration |
| Stem Cell Surface Marker Assessment | Using immunocytochemistry, flow cytometry, and alkaline phosphatase staining |
| In Vitro Embryoid Body (EB) Assay | Confirming pluripotency through 3D aggregate testing |
| Pluripotency and Tri-Lineage Differentiation Potential | Using the Applied Biosystems TaqMan hPSC Scorecard Panel to evaluate differentiation into ectoderm, mesoderm, and endoderm germ layers |
Coriell’s commitment to meticulous quality control ensures that every iPSC line meets the highest standards of reliability and reproducibility.
