GM15302
Somatic cell hybrid
Description:
CHROMOSOME 7 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 3 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(7)t(3;7)(3pter>3p21.1::7p13>7qter) in 96% of cells and 1, 2, 5, 8, 11, 12, 13, 15, 16, 18, 20, 21, and X
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Remarks
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| Remarks |
Produced by the fusion of human fibroblasts, 46,XY,t(3;7)(p21.1;p13), with RAG mouse cells; culture grown without selection; retains a human der(7)t(3;7)(p21.1;p13) in 96% of cells and other human chromosomes |
| Vortkamp A, Thias U, Gessler M, Rosenkranz W, Kroisel PM, Tommerup N, Kruger G, Gotz J, Pelz L, Grzeschik KH, A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p. Genomics11:737-43 1991 |
| PubMed ID: 1663489 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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