GM15297
Somatic cell hybrid
Description:
CHROMOSOME 2 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 6 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(2)t(2;6)(p23;p23) in 88% of cells and 13, 14, 15, 18, 19, 21, and 22
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Remarks
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| Remarks |
Produced by the fusion of human fibroblasts, 46,XY,t(2;6)(p23;p23), with A9 mouse cells; culture was grown without selection; retains a human der(2)t(2;6)(p23;p23) in 88% of cells & other human chromosomes |
| Grzeschik KH, Mayerova A, Bender K, Johannsmann R, Regional submapping of human chromosome 6 by the use of human-mouse somatic cell hybrids. Cytogenet Cell Genet22:412-4 1978 |
| PubMed ID: 752514 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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