GM14955
Somatic cell hybrid
Description:
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 12 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis before cell line submission to CCR
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ISCN
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Human/mouse somatic cell hybrid retains a human der(16)t(12;16)(q24;p11.2) in 68% of cells and a human 7 & 17
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line CY12; produced by the fusion of human cells with karyotype t(12;16) (q24;p11.2) with A9 mouse cells; grown in medium with azaserine & adenine; retains a human der(16)t(12;16)(q24;p11.2) in 68% of cells and human #7 and #17 |
| Callen DF, Baker E, Eyre HJ, Lane SA, An expanded mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet33:190-5 1990 |
| PubMed ID: 2095701 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 5 x 10-2mM |
| Supplement |
Azaserine 5 x 10-2mM |
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