GM14559
Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/Chinese hamster hybrid retaining a human der(18)t(3;18)(q27;q22.3) in 100% of cells and other human chromosomes
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Remarks
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 18p11.1-q11.2, D18S44, 18p11.31-p11.22, TYMS, and 18q12.1, TTR, and gave a negative result with a primer for 18q23, D18S1390. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3q13.1-q13.2, D3S1310, and 3q26.2-q27, GLUT2, and gave a positive result with a primer for 3q28-q29, D3S1311. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, 5q13.3-q14, HMGCR, and 5q34-q35, GABRA1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q36-qter, D7S550, and gave a positive result with a primer for 7pter-p22, D7S472. |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, and Xq21.3, DXS3. |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Line JH251; produced by the fusion of human lymphoblasts, t(3;18)(q27;q22.3), with Chinese hamster UCW206 cells; grown at 39C; 100% of cells retain a human der(18)t(3;18)(q27;q22.3) and other human chromosomes including #3 |
| Rojas K, Overhauser J, Sublocalization of 21 chromosome 18-specific microsatellite markers. Genomics18:169-71 1993 |
| PubMed ID: 8276412 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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