GM14415
Somatic cell hybrid
Description:
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 22 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Molecular characterization
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ISCN
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Human/hamster hybrid retaining a der(21) t(21;22)(q22.3;q11) in 88% of cells, #6 (76%), 13 (52%), 4 (40%), & 19 (80%)
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Remarks
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 21p11.2, D21S1278, 21q11, D21S120, 21q11.1, D21S236, and 21q21.2, AMYLOID. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 22q12.1-q13.1, D22S300, 22q13.31, KI-108, and 22q13.31-qter, DIA1, and gave a negative result with a primer for 22q11.2, IGLC2. |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line Raj5; produced by fusion of myelocytes from a CML pt, 46,XX,t(21;22)(q22; q11), with purine auxotrophic mutant CHO line, Ade-C; selected & grown in purinefree medium; retains hum der(21)t(21;22) (q22.3;q11) in 88% of cells |
| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| Graw SL, Gardiner K, Hall-Johnson K, Hart I, Joetham A, Walton K, Donaldson D, Patterson D, Molecular analysis and breakpoint definition of a set of human chromosome 21 somatic cell hybrids. Somat Cell Mol Genet21:415-428 1995 |
| PubMed ID: 8600569 |
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| Gardiner K, Watkins P, Munke M, Drabkin H, Jones C, Patterson D, Partial physical map of human chromosome 21. Somat Cell Mol Genet14:623-37 1988 |
| PubMed ID: 3194801 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 deficient Medium with 2mM L-glutamine or equivalent |
| Serum |
5% dialyzed fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 50mg/L |
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