GM14192
Somatic cell hybrid
Description:
CHROMOSOME 4 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a del(4)(p16.1) in 72% of cells, add(5)(p?) (96%), and #19 (88%)
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Remarks
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 19p13.2, LDLR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 9q22.3-q31, D9S53, and gave positive results with primers for 9p24, WI-7297, and 9q34-qter, D9S158. |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line HHW878; produced by the fusion of human cells with Chinese hamster cells; grown in medium with 0.2mM proline at 39C; retains a human del(4)(p16.1) in ??% of cells & other human chromosomes |
| Hayden MR, Hewitt J, Wasmuth JJ, Kastelein JJ, Langlois S, Conneally M, Haines J, Smith B, Hilbert C, Allard D, A polymorphic DNA marker that represents a conserved expressed sequence in the region of the Huntington disease gene. Am J Hum Genet42:125-31 1988 |
| PubMed ID: 2892395 |
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| Smith B, Skarecky D, Bengtsson U, Magenis RE, Carpenter N, Wasmuth JJ, Isolation of DNA markers in the direction of the Huntington disease gene from the G8 locus. Am J Hum Genet42:335-44 1988 |
| PubMed ID: 2963539 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 34.5mg/L |
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