GM13412
Somatic cell hybrid
Description:
CHROMOSOME 8 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster hybrid retaining human 8cen>8q22 inserted into a hamster chromo & other human chromos
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Remarks
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p23-p22, DEF1, 8q24, TG, 8q22.2, D8S270, 8p12, D8S87, 8p21-p11.2, LHRH, and 8q23-q24, D8S199, and gave a positive result with a primer for 8q11.22-q12, D8S260. M. Wagner, Research Triangle Park, NC, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8q11, MOS, and 8q13-q22.1, D8S286, and gave negative results with primers for 8p22, D8S258, 8p21.3-cen, D8S259, 8q22.2, D8S271, 8q22.3, D8S276, and 8q24.3-qter, D8S263. |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line 1HL35-5; human/Chinese hamster ovary somatic cell hybrid retaining human 8cen>q22 inserted into a hamster chromo in 100% of cells & other human chromos; grown in medium without selection |
| Wagner MJ, Ge Y, Siciliano M, Wells DE, A hybrid cell mapping panel for regional localization of probes to human chromosome 8. Genomics10:114-25 1991 |
| PubMed ID: 2045096 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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