GM13082
Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis and In situ hybridization
|
|
ISCN
|
Human/Chinese hamster hybrid retaining a human del(18)(q22.1) in 92% of cells, #17 (28%), & 22 (80%)
|
|
Remarks
|
|
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 18q23, MBP, and gave a positive result with a probe for 18q12.3, D18S6. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 18p11.31-p11.22, TYMS. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3p25, RAF1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Line JH295; produced by the fusion of human cells, 46,XX,del(18)(q22.1), with Chinese hamster UCW206 cells; grown in medium with 0.2mM proline at 39C; retains human del(18)(q22.1) in 92% of cells & other human chromosomes |
| Mahr RN, Moberg PJ, Overhauser J, Strathdee G, Kamholz J, Loevner LA, Campbell H, Zackai EH, Reber ME, Mozley DP, Brown L, Turetsky BI, Shapiro RM, Neuropsychiatry of 18q- syndrome. Am J Med Genet67(2):172-8 1996 |
| PubMed ID: 8723044 |
| |
| Strathdee G, Zackai EH, Shapiro R, Kamholz J, Overhauser J, Analysis of clinical variation seen in patients with 18q terminal deletions. Am J Med Genet59(4):476-83 1995 |
| PubMed ID: 8585568 |
| |
| van Kessel AG, Straub RE, Silverman GA, Gerken S, Overhauser J, Report and abstracts of the second international workshop on human chromosome 18 mapping. Doorwerth, the Netherlands, July 19-20, 1993. Cytogenet Cell Genet65:142-65 1994 |
| PubMed ID: 8222751 |
| |
| Kline AD, White ME, Wapner R, Rojas K, Biesecker LG, Kamholz J, Zackai EH, Muenke M, Scott CI Jr, Overhauser J, Molecular analysis of the 18q- syndrome--and correlation with phenotype. Am J Hum Genet52(5):895-906 1993 |
| PubMed ID: 8488839 |
| |
| Rojas K, Straub RE, Kurtz A, Feder M, Mewar R, Gilliam TC, Overhauser J, Identification and localization of microsatellite markers covering human chromosome 18. Genomics14:1095-7 1992 |
| PubMed ID: 1478651 |
| Split Ratio |
1:25 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
|
|