GM11779
Somatic cell hybrid
Description:
CHROMOSOME 8 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining human 8pter>8q22 & #4, 6, 7, 9, 10, 13, 14, 16, 19, 20, 21, 22, 22, & X
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Remarks
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
M. Wagner, Research Triangle Park, NC, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p22, D8S258, 8p21.3-cen, D8S259, 8q11, MOS, 8q13-q22.1, D8S286, 8q22.2, D8S271, and 8q22.3, D8S276, and gave a negative result with a primer for 8q24.3-qter, D8S263. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8q23-q24, D8S199, and 8q24, TG, and gave positive results with primers for 8p12, D8S87, 8p23-p22, DEF1, 8p21-p11.2, LHRH, 8q11, MOS, 8q11.22-q12, D8S260, and 8q22.2, D8S270. |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line XVIII-23H-a; produced by the fusion of human fibros, 46,X,del(X), with HPRTdef Chinese hamster cells; grown without selection; retains human 8pter>8q22 translocated to a hamster chromosome in 86% of cells & other human chromosomes |
| White RA, Dowler LL, Pasztor LM, Gatson LL, Adkison LR, Angeloni SV, Wilson DB, Assignment of the transcription factor GATA4 gene to human chromosome 8 and mouse chromosome 14: Gata4 is a candidate gene for Ds (disorganization). Genomics27:20-6 1995 |
| PubMed ID: 7665171 |
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| Wagner MJ, Ge Y, Siciliano M, Wells DE, A hybrid cell mapping panel for regional localization of probes to human chromosome 8. Genomics10:114-25 1991 |
| PubMed ID: 2045096 |
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| Floyd-Smith G, De Martinville B, Francke U, An expressed beta-tubulin gene, TUBB, is located on the short arm of human chromosome 6 and two related sequences are dispersed on chromosomes 8 and 13. Exp Cell Res163:539-48 1986 |
| PubMed ID: 3007184 |
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| Yang-Feng TL, Opdenakker G, Volckaert G, Francke U, Human tissue-type plasminogen activator gene located near chromosomal breakpoint in myeloproliferative disorder. Am J Hum Genet39:79-87 1986 |
| PubMed ID: 3092643 |
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| Francke U, Random X inactivation resulting in mosaic nullisomy of region Xp21.1---- p21.3 associated with heterozygosity for ornithine transcarbamylase deficiency and for chronic granulomatous disease. Cytogenet Cell Genet38:298-307 1984 |
| PubMed ID: 6510024 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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