GM10889
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis and Southern blot hybridization
|
|
ISCN
|
Mouse/human hybrid retaining a human del(17) chromosome in 84% of cells
|
|
Remarks
|
|
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 10q24qter, PLAU. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 14q32.1q32.3, D14S13. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 17p13.1, TP53, 17q21.3-q23, MPO1, 17q25-qter, D17S784, and 17q, GAS. |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Line DHA4; produced by fusing human cells, 46,XY,del(17)(p11.2p11.2), with TK-deficient Cl1D mouse cells; selected in HAT medium; retains human del(17) in 84% of cells & no other intact human chromosomes |
| Moran JL, Johnston SH, Rauskolb C, Bhalerao J, Bowcock AM, Vogt TF, Genomic structure, mapping, and expression analysis of the mammalian Lunatic,
Manic, and Radical fringe genes. Mamm Genome10(6):535-41 1999 |
| PubMed ID: 10341080 |
| |
| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
| |
| Ishizuka T, Ahmad I, Kita K, Sonoda T, Ishijima S, Sawa K, Suzuki N, Tatibana M, The human phosphoribosylpyrophosphate synthetase-associated protein 39 gene (PRPSAP1) is located in the chromosome region 17q24-q25. Genomics33:332-4 1996 |
| PubMed ID: 8660991 |
| |
| Flejter WL, Bennett-Baker P, Barcroft CL, Kiousis S, Chamberlain JS, Region-specific cosmids and STRPs identified by chromosome microdissection and FISH. Genomics25:413-20 1995 |
| PubMed ID: 7789975 |
| |
| Polymeropoulos MH, Torres R, Yanovski JA, Chandrasekharappa SC, Ledbetter DH, The human corticotropin-releasing factor receptor (CRHR) gene maps to chromosome 17q12-q22. Genomics28:123-4 1995 |
| PubMed ID: 7590738 |
| |
| Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ, High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3). Genomics20:249-57 1994 |
| PubMed ID: 8020972 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
|
|