GM08686
LCL from B-Lymphocyte
Description:
BURKITT LYMPHOMA; BL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
dbGaP |
| Class |
Heritable Cancer Syndromes and other Cancers |
| Alternate IDs |
GM17348 [BURKITT LYMPHOMA; BL] |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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Black/African American
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Ethnicity
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AFRICAN
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX,t(8;14)(8pter->8q24.1::14q32.2->14qter;14pter->14q32.2::8q24.1->8qter)[13]/46,XX,t(8;14),)(8pter->8q24.1::14q32.2->14qter;14pter->14q32.2::8q24.1->8qter)add(4)(p16),add(14)(p13)[2]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Cytogenetics |
Chromosome 14: TRANSLOCATION Breakpoint 14q32 t(8;14)14q32 |
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Chromosome 8: TRANSLOCATION Breakpoint 8q24 t(8;14)8q24 |
| Remarks |
Line IARC/BL16; stage III abdominal lymphoma; African; 46,XX,t(8;14)(q24.1; q32.3),4p+,14p+; 16% of cells do not have the 4p+ and 14p+ marker chromosomes; pseudodiploid |
| Bernheim A, Berger R, Lenoir G, Cytogenetic studies on Burkitt's lymphoma cell lines. Cancer Genet Cytogenet8:223-9 1983 |
| PubMed ID: 6825067 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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