NA11908
DNA from Somatic cell hybrid
Description:
CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
DNA from Somatic cell hybrid
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/Chinese hamster hybrid retaining human 3pter>3q27(28) translocated to a Chinese hamster chromosome
|
|
Remarks
|
|
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 21q11, D21S26, and 21q22.1q22.2, D21S17. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 3p25, RAF1, and gave a negative result with a probe for 3q26.2qter, TFRC. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, 3p25p24.2, D3S1304, 3q13.1-q13.2, D3S1310, 3q22-q24, D3S1764, and 3q26.2a negative result with a primer for 3q28-q29, D3S1311. q27, GLUT2, and gave a negative result with a primer for 3q28-q29, D3S1311. |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Line R148-3 was derived from hybrid 3141 from the fusion of normal hum lymphs with Chinese hamster ovary cells (Urd-C mutant); grown in F12 med with 5% macroserum; retains 3pter>q27(28) translocated to a hamster chromo in 92% of cells |
| Bailey A, Norris AL, Leek JP, Clissold PM, Carr IM, Ogilvie DJ, Morrison JF, Meredith DM, Markham AF, Yeast artificial chromosome cloning of the beta-catenin locus on human chromosome 3p21-22. Chromosome Res3:201-3 1995 |
| PubMed ID: 7780664 |
| |
| Drabkin H, Wright M, Jonsen M, Varkony T, Jones C, Sage M, Gold S, Morse H, Mendez M, Erickson P, Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3. Genomics8:435-46 1990 |
| PubMed ID: 2286371 |
|
|