AG08606
Fibroblast from Skin, Arm
Description:
ANEUPLOID CHROMOSOME NUMBER - NON-TRISOMIC
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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45,X
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
18 |
| Passage Frozen |
9 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor was diagnosed as Turner syndrome based on karyotype analysis of skin fibroblasts prior to submission to the Repository and from features of short stature, short neck and questionable low hairline. Patient also has some developmental delay. The culture was initiated from explants of minced skin tissue taken ante-mortem from the right forearm. The cell morphology is fibroblast-like. The karyotype is 45,X. A culture from the right gonad of same donor is AG08607. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| San Roman AK, Godfrey AK, Skaletsky H, Bellott DW, Groff AF, Harris HL, Blanton LV, Hughes JF, Brown L, Phou S, Buscetta A, Kruszka P, Banks N, Dutra A, Pak E, Lasutschinkow PC, Keen C, Davis SM, Tartaglia NR, Samango-Sprouse C, Muenke M, Page DC, The human inactive X chromosome modulates expression of the active X chromosome Cell genomics3:100259 2022 |
| PubMed ID: 36819663 |
| Cumulative PDL at Freeze |
18 |
| Passage Frozen |
9 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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