AG07955
Fibroblast from Skin, Arm
Description:
XERODERMA PIGMENTOSUM: COMPLEMENTATION GROUP UNDETERMINED
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Heritable Diseases |
|
Biopsy Source
|
Arm
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Arm
|
|
Race
|
White
|
|
Ethnicity
|
FINNISH
|
|
Family Member
|
3
|
|
Relation to Proband
|
brother
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XY
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
19 |
| PDL at Freeze |
9 |
| Passage Frozen |
7 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
The Finnish donor is clinically affected with features of photophobia, freckling, atrophy and telangiectasia of facial skin, severe neurological abnormalities with low IQ, and growth deficiency. The family history is positive with two similarly affected sibs. The skin biopsy was taken antemortem on 11/30/84 from the inner aspect of the upper left arm. The culture was initiated using explants of minced skin tissue. The cell morphology is fibroblast-like. Culture grows slowly. A lymphoblast culture from same donor is AG07954A. |
| PDL at Senescence |
19 |
| Cumulative PDL at Freeze |
9.02 |
| Passage Frozen |
7 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagles Minimum Essential Medium with Earle's salts:Dulbecco's modified MEM with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|