AG07167
Fibroblast from Skin, Shoulder
Description:
ANEUPLOID CHROMOSOME NUMBER - TRISOMY 18
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Shoulder
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Shoulder
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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47,XX,+18
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The donor was diagnosed as trisomy 18 and had delayed physical and psychomotor development since birth. She developed Wilms tumor at 11 years of age. The biopsy was taken ante-mortem from skin of the deltoid area. The culture was initiated on 10/20/83 using explants of minced skin tissue. The cell morphology is fibroblast-like. Karyotype is 47,XX,+18. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Kim SY, Lee SM, Kim SM, Kim BJ, Koo JN, Oh IH, Oh S, Park CW, Jun JK, Lim JH, Ryu HM, Park JS, Novel method of real-time PCR-based screening for common fetal trisomies BMC medical genomics14:195 2020 |
| PubMed ID: 34330281 |
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| Karayalcin G, Shanske A, Honigman R, Wilms' tumor in a 13-year old girl with trisomy 18. Am J Dis Child135:665-6 1981 |
| PubMed ID: 6264780 |
| Cumulative PDL at Freeze |
5 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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