AG04407
Fibroblast from Skin, Chest
Description:
CYSTIC FIBROSIS; CF
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Heritable Diseases |
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Biopsy Source
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Chest
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Chest
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Remarks |
Donor was clinically affected and had a positive sweat test. Donor died of exacerbation of pulmonary cystic fibrosis with heart failure and bronchopneumonia. The biopsy was taken postmortem at autopsy from skin of the chest on 10/23/80. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. Culture grows slowly. |
| Passage Frozen |
3 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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