AICS-0023

AICS-0023 iPSC from Stem cell

Description:

MONO-ALLELIC mEGFP-TAGGED TJP1 WTC iPSC LINE (TAG AT N-TERM)

Overview

Repository

Allen Cell Collection

Protocols

Prior to thawing a vial from the Allen Cell Collection please determine which vial type purchased (see vials images in Protocol linked below).

AICS SOP WTC Cell Culture AICS SOP WTC Cell Plating On Glass For Imaging

Cell Type

Stem Cell

Cell Subtype

Induced pluripotent stem cell

Sample Source

iPSC from Stem cell

Species

Homo sapiens

Common Name

Human

Certificate of Analysis

Download Certificate Download STR Analysis

Remarks

Gene: TJP1, Protein: Tight junction protein ZO-1, Tagged Alleles: Mono, Structure: Tight junctions, Fluorescent Tag: mEGFP, Tagged Location: N-terminus, Clone: 20, Passage: 28

More Data

More data and images on this line are available at the Allen Institute for Cell Science's data portal. You can also view a short video demonstrating how to single cell passage AICS lines.

Parental line

This Allen Cell line was derived from the WTC parental line released by the Conklin Laboratory at the J. David Gladstone Institute (available through Coriell as GM25256)

Characterizations

Gene

TJP1

Chromosomal Location

15q13.1

Phenotypic Data

Remarks

Gene: TJP1, Protein: Tight junction protein ZO-1, Tagged Alleles: Mono, Structure: Tight junctions, Fluorescent Tag: mEGFP, Tagged Location: N-terminus, Clone: 20, Passage: 28

Publications

Pilz RA, Skowronek D, Mellinger L, Bekeschus S, Felbor U, Rath M, Endothelial Differentiation of International journal of molecular sciences24: 2022

PubMed ID: 36835400

Legier T, Rattier D, Llewellyn J, Vannier T, Sorre B, Maina F, Dono R, Epithelial disruption drives mesendoderm differentiation in human pluripotent stem cells by enabling TGF-ß protein sensing Nature communications14:349 2021

PubMed ID: 36681697

Culture Protocols

Supplement

Pricing

Commercial:

Academic &
Non-profit:

$750.00USD

Description:

MONO-ALLELIC mEGFP-TAGGED TJP1 WTC iPSC LINE (TAG AT N-TERM)

Affected:

No Data

Sex:

No Data

Age:

No Data

Overview

Characterizations

Phenotypic Data

Publications

Culture Protocols